Effects of P. amaryllifolius extract on MDA-MB-231 cell DNA fragmentation. ( a) P. amaryllifolius altered cell cycle distributions (%) but not sub-G0/G1 population in treated breast cancer MDA-MB-231 cells as compared to controls after 24 hr of treatment in cell cycle PI study. ( b),( c) P. amaryllifolius induced subG0/G1 population of MDA-MB-231 cell after 48 and 72 hr treatment exposure detected by cell cycle PI staining assay. ( d) P. amaryllifolius increased cell population of early and secondary apoptosis after 48 hr of treatment in AnnexinV/PI study. ( e), ( f) P. amaryllifolius induced DNA fragmentation of MDA-MB-231 cell after 48 and 72 hr treatment exposure detected by TUNEL assay. Values are presented as means (n = 3) ± S.E. *signified (p < 0.05).