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Figure 8 | BMC Complementary and Alternative Medicine

Figure 8

From: Protective effect of cactus cladode extract against cisplatin induced oxidative stress, genotoxicity and apoptosis in balb/c mice: combination with phytochemical composition

Figure 8

Immunoblot (a) and densitometric (b) analysis of bcl2 in kidney of control and treated animals. The protein was separated on 12% SDS-PAGE and blotted with anti-bcl2 antibody. The intensity of the protein band was scanned by densitometry. Some modifications are made to blots by adjusting the luminosity and contrast of the protein bands to better understand the effects of CDDP and CCE (unmodified versions of Western blot have been included as an Additional file 1: Figure S1). Results are significantly different as compared to controls (p<0.005). The results are representative of eight independent experiments: (1) Animals treated by 100 μl H2O (2) Animals treated by CCE 50 mg/Kg b.w (3) Animals treated 15 days by CDDP 100 μg/Kg b.w (4) Animals treated by CCE 50 mg/Kg b.w before CDDP 100 μg/Kg b.w exposure for 15 days treatment (5) Animals treated by CCE 50 mg/Kg b.w after CDDP 100 μg/Kg b.w exposure for 15 days treatment (6) Animals treated 30 days by CDDP 100 μg/Kg b.w (7) Animals treated by CCE 50 mg/Kg b.w before CDDP 100 μg/Kg b.w exposure for 30 days treatment (8) Animals treated by CCE 50 mg/Kg b.w after CDDP 100 μg/Kg b.w exposure for 30 days treatment.

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