IL-4, IL-10, IFN-γ and TGF-β synthesis by spleen cells. For the cytokine immunoassay, spleen cells (5x10
) were incubated for 24 h with RPMI-1640 culture medium. Cell-free supernatant was assayed for IL-4, IL-10, IFN-γ and TGF-β by ELISA according to the manufacturer’ instructions. P (tumor-bearing mice treated with pulchellin at 0.75 μg/kg of body weight), T (tumor-bearing mice treated with saline solution) and N (untreated healthy mice). One-way ANOVA with Tukey’s post test was performed. * p < 0.05, ** p < 0.01 vs. N group; # p < 0.05, ## p < 0.01 and ### p < 0.001 vs. T group.