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Figure 2 | BMC Complementary and Alternative Medicine

Figure 2

From: Immunological response in mice bearing LM3 breast tumor undergoing Pulchellin treatment

Figure 2

IL-6 and TNF-α synthesis by PEC cells. For the cytokine immunoassays, adherent cells (5x10 6 cells ml -1 ) were incubated for 24 h with RPMI-1640 culture medium. Cell-free supernatant was assayed for IL-6 and TNF-α by ELISA according to the manufacturer’ instructions. P (tumor-bearing mice treated with pulchellin at 0.75 μg/kg of body weight), T (tumor-bearing mice treated with saline solution) and N (untreated healthy mice). One-way ANOVA with Tukey’s post test was performed. ** p < 0.01, *** p < 0.001 vs. N group; ## p < 0.01 vs. T group.

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