IL-6 and TNF-α synthesis by PEC cells. For the cytokine immunoassays, adherent cells (5x10
) were incubated for 24 h with RPMI-1640 culture medium. Cell-free supernatant was assayed for IL-6 and TNF-α by ELISA according to the manufacturer’ instructions. P (tumor-bearing mice treated with pulchellin at 0.75 μg/kg of body weight), T (tumor-bearing mice treated with saline solution) and N (untreated healthy mice). One-way ANOVA with Tukey’s post test was performed. ** p < 0.01, *** p < 0.001 vs. N group; ## p < 0.01 vs. T group.