NO Synthesis by Peritoneal Macrophages. Adherent cells (5x10
) were incubated for 24 h with RPMI-1640 culture medium. Cell-free supernatant was mixed with the Griess reagent and the resulting reaction was read in a spectrophotometer. P (tumor-bearing mice treated with pulchellin at 0.75 μg/kg of body weight), T (tumor-bearing mice treated with saline solution) and N (untreated healthy mice). One-way ANOVA with Tukey’s post test was performed. ** p < 0.01 vs. N group; # p < 0.05 vs. T group.