Figure 1From: Pharmacological modulation of histone demethylase activity by a small molecule isolated from subcritical water extracts of Sasa senanensis leaves prolongs the lifespan of Drosophila melanogaster Purification and identification of PA. (A) Purification of antioxidative compounds from S. senanensis leaves. Subcritical water extracts were prepared from the leaves and fractionated by HPLC with MeOH/H2O (17:83). The SOSA was assessed for each fraction, and the highest value detected in fraction 4. (B) Purification of the compound by HPLC. Fraction 4 was further fractionated, and fraction 4-II found to contain the highest SOSA. (C) EI-MS spectra data of purified PA, which was identified using the SDBS. (D–F) HPLC chromatograms of fraction 4-II (D), synthetic PA (E), and a mixture of fraction 4-II and synthetic PA (F), in which the two compounds were co-eluted as a single peak. (G) Chemical structure of PA.Back to article page