Af extract inhibits the peroxyl radical - induced oxidation of DCFH in primary microglial cells. Microglial cells were incubated for 1 h with Af extract. They were then preloaded with DCF-DA for 30 min and washed with PBS, after which, 0.6 mM ABAP was added and ROS levels were measured at the indicated time points. Each point represents mean ± SEM of 2 experiments (n = 8). A. Af extract at 50 μg/mL. B. ROS production was measured 22 h after the addition of ABAP. * p < 0.05; ** p < 0.001.