Effect of RM on FAS and ACS1. 3T3-L1 pre-adipocytes 2 days after confluence were induced to differentiate in the presence of RM (0-300 μg/ml). At day 9 adipocytes RNA was isolated and RT-PCR for mRNA expression of (A) fatty acid synthase (FAS) and (B) acyl-coA synthetase (ACS1) was performed. Ratio is calculated against β-actin represented as arbitary units of integrated density value (IDV). Each experiment was repeated twice in duplicate and results are depicted as mean ± SD, statistical significance was calculated (letters that differ indicate, p ≤ 0.05).