Figure 2

Effect of RM on adipogenic transcription factors. 3T3-L1 pre-adipocytes 2 days after confluence were induced to differentiate in the presence of RM (0-300 μg/ml). At day 9 adipocytes RNA was isolated and RT-PCR for mRNA expression of (A) PPAR-γ, (B) SREBP-1 and (C) c/EBP-α was performed as described in Materials and Methods. Ratio is calculated against β-actin represented as arbitary units of integrated density value (IDV). Each experiment was repeated twice in duplicate and results are depicted as mean ± SD, statistical significance was calculated (letters that differ indicate, p ≤ 0.05).