Figure 5

Cartilage matrix status. HLM inhibited the IL-1β-induced release of GAG from human cartilage explants in vitro. Cartilage pieces (26.7 mg ± 1.3) were incubated with medium alone or medium containing either IL-1β alone or in combination with HLM (1 and 5 μg/ml) for 7 days. Total GAG release from cartilage explants in culture supernatants was quantified by using Proteoglycan detection kit and values are derived from standard curve. Value represents Mean ± SD of three different experiments repeated in duplicate. * p < 0.05.