Figure 2

Effect of HLM ( Uncaria tomentosa ., Boswellia spp ., Lepidium meyenii and L-Leucine ) on the IL-1β-induced gene expression of inducible nitric oxide synthase (iNOS) (A) and on the production of nitric oxide (NO) (B) in human OA chondrocytes. Chondrocytes were pretreated for 2 h with HLM (1-5 μg/ml) and then stimulated or not stimulated with IL-1β (5 ng/ml) for 24 h. Relative gene expression of iNOS was determined by Real-time PCR using GAPDH as endogenous control and compared with un-stimulated control. Corresponding culture supernatants were analyzed for total NO levels. Values represent Mean ± SE of three different experiments run in duplicate. * p < 0.05.