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Table 3 Minimal inhibitory concentration (MIC)/minimum bactericidal or fungicidal concentration (MBC or MFC) of test substances (μg/ml)

From: Antimicrobial and antioxidant properties of methanol extract, fractions and compounds from the stem bark of Entada abyssinica Stend ex A. Satabie

Microorganisms Parameters Test samplesa
   ME HF EF n B AR 1 2 3 4 Ref
Gram (-) bacteria            
Pseudomonas MIC 195.3 24.4 781.2 390.6 781.2 39.0 9.7 9.7 312.5 1.5
aeruginosa MBC 781.2 390.6 nab na na 156.2 19.5 9.7 > 312.5 12.5
Proteus MIC 195.3 390.6 390.6 781.2 na 156.2 19.5 39.0 na 0.3
mirabilis MBC 781.2 na 390.6 na na 156.2 39.0 156.2 na 1.5
Shigella flexneri MIC 781.2 48.8 390.6 24.4 390.6 78.1 39.0 39.0 na 0.2
  MBC na 390.6 390.6 195.3 na 156.2 156.2 78.1 na 6.2
Klebsiella MIC 781.2 24.4 97.6 781.2 na 78.1 19.5 78.1 na 0.2
pneumoniae MBC na 195.3 390.6 na na 156.2 39.0 156.2 na 3.1
Salmonella typhi MIC 781.2 390.6 97.6 781.2 na 156.2 39.0 78.1 na 0.3
  MBC na na 390.6 na na 312.5 156.2 156.2 na 1.5
Escherichia coli MIC 781.2 24.4 97.6 24.4 na 78.1 39.0 156.2 312.5 0.2
  MBC na 390.6 390.6 390.6 na 312.5 312.5 156.2 > 312.5 1.5
Gram (+) bacteria  
Enterococcus MIC 195.3 390.6 390.6 781.2 na 78.1 39.0 78.1 na 0.2
faecalis MBC na 781.2 781.2 na na 312.5 312.5 156.2 na 1.5
Staphylococcus MIC 390.6 195.3 97.6 781.2 390.6 78.1 39.0 78.1 na 1.5
aureus MBC na na 390.6 na na 39.0 156.2 156.2 na 12.0
Yeast  
C. albicans MIC na na 195.2 390.6 195.3 312.5 312.5 78.1 na 0.01
ATCC 2091 MFC na na 195.3 390.6 1953 312.5 312.5 156.2 na 0.08
C. albicans MIC 781.2 781.2 195.3 195.3 195.3 312.5 156.2 156.2 312.2 0.01
ATCC9002 MFC na na 781.2 195.3 390.6 312.5 156.2 156.2 > 312.5 0.08
C. albicans MIC 195.3 390.6 195.3 390.6 195.3 156.2 312.5 78.1 na 0.02
ATCC 24433 MFC 781.2 na 195.3 na 195.3 312.5 312.5 156.2 na 0.02
Candida MIC 781.2 781.2 195.3 390.6 390.6 312.5 156.2 78.1 na 0.01
parapsilosis MFC na 390.6 781.2 390.6 781.2 312.5 156.2 156.2 na 0.08
Candida MIC 195.3 na 48.8 390.6 195.3 312.5 312.5 78.1 na 0.04
tropicalis MFC 781.2 na 390.6 781.2 195.3 312.5 312.5 156.2 na 0.08
Candida krusei MIC na 781.2 781.2 390.6 195.3 312.5 312.5 78.1 na 0.04
  MFC na na na 390.6 195.3 312.5 312.5 156.2 na 0.04
Candida glabrata MIC na 781.2 195.3 390.6 390.6 78.1 312.5 78.1 na 0.02
  MFC na na 195.3 na 781.2 78.1 312.5 156.2 na 0.02
Candida MIC 781.2 390.6 195.3 195.3 195.3 156.2 312.5 78.1 na 0.01
lusitaniae MFC na 781.2 390.6 195.3 195.3 156.2 312.5 78.1 na 0.08
Cryptococcus MIC 390.6 781.2 97.6 390.6 195.3 195.3 78.1 78.1 na 1.00
neoformans MFC na na 781.2 390.6 195.3 312.5 156.2 78.1 na 1.00
Candida MIC 781.2 781.2 195.3 390.6 390.6 312.5 156.2 78.1 na 0.01
guillermondi MFC na na na na 781.2 312.5 156.2 156.2 na 0.08
  1. aME: methanol extract; HF: n- hexane fraction; EF: Ethyl acetate fraction; nB: n-butanol fraction; AR: Aqueous residue; 1: (5S,6R,8aR)-5-(carboxymethyl)-3,4,4a,5,6,7,8,8a-octahydro-5,6,8a-Trimethylnaphthalenecarboxylic acid; 2: methyl 3,4,5-trihydroxybenzoate; 3: benzene-1,2,3-triol; 4: 2,3-dihydroxypropyltriacontanoate and Ref (reference drugs): ciprofloxacin for bacteria and nystatin for yeasts.
  2. bna: not active at concentrations upto 781.2 μg/ml.