Figure 3

NF-κB activity of HCC cell lines in the presence of L.obtusiloba extract. HCC cell lines were transfected with a NF-κB/luciferase reporter plasmid and allowed to adhere for 20 h before being treated with 10 μg/ml TNFα, 100 μg/ml L.obtusiloba extract or a combination of both. Untreated cells served as negative controls. After additional 24 h, cells were lysed and luciferase activity was determined. Shown are the mean values ± SD of four parallel measurements. *P < 0.05, ***P < 0.001.