Figure 5

Effect of SR10 on cell cycle distribution of PDGF-BB-treated A7r5 cells. After starving with 1% fetal bovine serum for 24 hours, A7r5 cells were treated with PDGF-BB (25 ng/ml) in the absence or presence of SR10 for 24 hours. Then, the cells were stained with PI and cell cycle distribution was analyzed by flow cytometry. Three experiments were performed and similar results were observed. This figure showed the result of one representative experiment (A). The expression level of cyclin D1 was detected by Western blot. The figure is a representative of three independent experiments with similar results. The quantitation of cyclin D1 was performed by analyzing three sets of data by One-way ANOVA. Treatment of 1.25, 2.5 or 5 mg/ml of SR10 all suppressed the expression of cyclin D1 which was induced by PDGF. Statistical significance was indicated by ** p < 0.01 or *** p < 0.001 (B). The expression level of ERK1/2 was detected by Western blot. The figure is a representative of three independent experiments with similar results. The quantitation of ERK1/2 was performed by analyzing three sets of data by One-way ANOVA. Treatment of 1.25, 2.5 or 5 mg/ml of SR10 all suppressed the expression of cyclin D1 which was induced by PDGF (data shown in red boxes). Statistical significance was indicated by *** p < 0.001 (C).