Figure 4
In vivo imaging of mice vaginally infected with Candida albicans and treated with essential oils. The vaginal lumen of mice under pseudoestrus condition were infected for 2 consecutive days with 10 μl of a 109 cell/ml suspension of Candida albicans gLUC59 (first three mice of each group) or control strain (fourth mouse of each group) and treated with the different essential oils 2 h before the first challenge and then every two days. After 5, 7, 9, 15 and 21 days post-infection mice were treated intravaginally with 10 μg of coelenterazine and imaged in the IVIS-200TM imaging system under anaesthesia with 2.5% isoflurane. Total photon emission from vaginal areas within the images (Region Of Interest, ROI) of each mouse was quantified with Living ImageR software package. Data are from one of three experiments with similar results.