Figure 2

Effect of essential oil on yeast and hyphae survival. gLUC59 Candida albicans yeast cells (A) and preformed hyphae (B) were treated with different concentrations of essential oils (EOMS, TTO and JO) for 24 h. After incubation 10 μl of coelenterazine substrate (1 mg/ml) was added to each well and samples were read using a luminometer. Results are expressed as percentage of yeast or hyphae survival and indicated as mean ± SD of triplicate samples are from one of three experiments with similar results. The statistical significance was evaluated with the Student's t-test (two-tailed). * P-values of < 0.05 were considered significant. The effect on preformed hyphae was microscopically examined after 24 h of treatment with essential oil (C). Original magnification of 20x or 40x is indicated in the micrographs. The results are representative of one of three independent experiments.