Figure 5

(A) Represents effect of flavonoidal fraction on peroxynitrite levels in mouse islets exposed to streptozotocin. Peroxynitrite levels were quantified by fluorescent probe dihydrorhodamine 123. Values are expressed as ± SEM in triplicates where • = p < 0.01, ■ = p < 0.001, ♦ = p < 0.0001 when control group was compared with rest of the groups and α = p < 0.01, β = p < 0.01, δ = p < 0.01 when diabetic group was compared to rest of the groups (B) Represents effect of flavonoidal fraction on NO levels in mouse islets exposed to streptozotocin. Nitric oxide levels were quantified by fluorescent probe DAF-FM. Values are expressed as ± SEM in triplicates where • = p < 0.01, ■ = p < 0.001, ♦ = p < 0.0001 when control group was compared with rest of the groups and α = p < 0.01, β = p < 0.01, δ = p < 0.01 when diabetic group was compared to rest of the groups (C) Represents effect of flavonoidal fraction on mitochondrial membrane potential (MMP) in mouse islets exposed to streptozotocin. MMP levels were quantified by fluorescent probe Rhodamine 123. Values are expressed as ± SEM in triplicates where • = p < 0.01, ■ = p < 0.001, ♦ = p < 0.0001 when control group was compared with rest of the groups and α = p < 0.01, β = p < 0.01, δ = p < 0.01 when diabetic group was compared to rest of the groups (D) Represents effect of flavonoidal fraction on lipid peroxidation levels (LPO) in mouse islets exposed to streptozotocin. Lipid peroxidation was measured by using thiobarbituric acid reagent. Values are expressed as ± SEM in triplicates where • = p < 0.01, ■ = p < 0.001, ♦ = p < 0.0001 when control group was compared with rest of the groups.