Figure 2

Apoptosis detected by hematoxylin and eosin (H & E), propidium iodide (PI), and TUNEL staining. PC-3M-1E8 cells were treated with 10 μg/ml TC extract for 24 h. Cells were processed for H & E staining (top panel), PI staining (middle panel) and TUNEL reaction (bottom panel). Apoptotic cells showed condensed and fragmented nuclei and positive TUNEL reaction. The H & E and TUNEL staining were observed by optical microscopy (magnification, × 200). The results of PI analysis were observed by a confocal microscopy. Scale line = 20 μm.