Cytotoxicity assay for different cells cultured with different concentrations of DWE. (A) NIH/3T3 cell survival curves from the CCK-8 assay. The negative control was untreated cells, and the blank control contained the Cell Counting Kit-8 reagent and no cells. The curve represents the average of three independent experiments with SD bars. (B) PBMCs were incubated at 37°C in 5% CO2 overnight in media supplemented with IL-2 at a concentration of 100 IU/ml. The cells were then exposed to the DWE for 24 h, and aliquots of cells were stained with trypan blue dye to determine the total numbers of cells as well as viable cells. PBMC cells, cultured in the absence of DWE were used as control. These data represent three independent experiments.