Construction of retroviral and HIV-1 replication reporter vector system. (A) The pLNCX2 -EGFP vector, constructed by inserting the EGFP gene into the multiple cloning site of the pLNCX2 plasmid, a retroviral vector contains elements derived from Moloney murine leukemia virus (MoMuLV) and Moloney murine sarcoma virus (MoMuSV). The HIV-1 based lentiviral vector pLL3.7 is commercially available, and EGFP is driven by the CMV promoter. (B) The development of stable RetroPackPT67 packaging cells encoding the EGFP genes, which was used as the EGFP virus-producing cell line. (C) Outline of the anti-HIV-1 activity assay for DWE.