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Table 6 Effect of RHP and GLGPG on gene expression in NHAC-kn cells

From: Rose hip and its constituent galactolipids confer cartilage protection by modulating cytokine, and chemokine expression

Gene CT a IL-1β IL-1β + RHP (250 mg/L) IL-1β + GLGPG (9.7 mg/L)
   fold change fold change p valueb fold change p valueb
MMP-1 29.5 22.8 10.4 0.008 20.0 0.418
MMP-2 23.4 0.8 0.6 0.249 0.8 0.332
MMP-3 28.1 167 89 0.048 128 0.135
MMP-9 29.6 7.2 6.0 0.025 5.0 0.203
MMP-13 28.1 2.2 1.3 0.048 2.0 0.135
ADAMTS-4 29.5 20.0 21.7 0.460 14.6 0.103
ADAMTS-5 21.7 1.3 0.9 0.011 1.7 0.148
TIMP-1 17.2 1.2 0.9 0.052 1.1 0.215
Aggrecan 28.8 1.0 0.8 0.196 1.1 0.340
Collagen 1 18.0 0.7 0.5 0.05 0.8 0.30
COL2A1 22.2 1.0 0.5 0.137 1.2 0.325
MIP-2 29.9 441 110 0.082 626 0.147
MIP-3α 30.0 481 271 0.007 436 0.126
CCL5/RANTES 26.7 87 42.0 0.009 54 0.046
IL-8 26.3 799 168 0.002 760 0.356
CXCL10/IP-10 ndc      
IL-1β 31.3 1078 530 0.002 505 0.085
IL-6 29.8 2011 1048 0.069 1927 0.226
IL-1Ra 28.4 1.2 0.6 0.107 1.2 0.272
IL-1RI 22.6 0.9 0.5 < 0.001 0.8 0.301
TNF-α ndc      
iNOS ndc      
COX-2 26.58 133 48.3 0.11 186 0.03
LIF 23.8 35.2 19.2 0.003 28.9 0.239
  1. IL-1β -stimulated cells were cultured with 31.3 - 1000 mg/L RHP or 1.2 - 38.7 mg/L of GLGPG for 4 h and gene expression was quantified by RT-PCR. Fold changes were calculated as indicated in Materials and Methods. Only the effects of 250 mg/L RHP and 9.7 mg/L GLGPG are shown.
  2. aCT = cycle threshold (CT < 22 = high basal gene expression; 23 < CT < 30 = intermediate basal gene expression; CT > 30 = low basal gene expression).
  3. b p value = significance value between (IL-1β)- and (IL-1β + substance) -treatment
  4. cnd = not done