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Table 5 Effect of RHP and GLGPG on gene expression in human chondrosarcoma SW1353 cells

From: Rose hip and its constituent galactolipids confer cartilage protection by modulating cytokine, and chemokine expression

Gene CT a IL-1β IL-1β + RHP (250 mg/L) IL-1β + GLGPG (9.7 mg/L)
   fold change fold change p valueb fold change p valueb
MMP-1 27.2 6.3 3.6 0.002 4.5 0.019
MMP-2 22.3 0.8 0.6 0.006 1.0 0.142
MMP-3 32.7 182.7 80.6 < 0.001 137.0 0.003
MMP-9 31.8 6.3 7.1 0.285 10.7 0.001
MMP-13 28.4 17.7 8.9 < 0.001 12.7 0.005
ADAMTS-4 35.3 10.7 9.8 0.136 9.6 0.026
ADAMTS-5 31.1 0.8 0.7 0.476 1.4 0.006
TIMP-1 21.2 1.4 1.2 0.195 1.9 0.007
Aggrecan 32.0 1.8 1.3 0.017 3.7 < 0.001
Collagen I 19.2 1.1 0.8 0.028 1.8 0.001
COL2A1 31.9 0.8 0.6 0.001 0.9 0.526
MIP-2 40.2 1169 474 < 0.001 1626 0.006
MIP-3α 38.5 19642 12001 < 0.001 20822 0.437
CCL5/RANTES 42.9 35920 27081 0.048 41476 0.353
IL-8 34.5 12022 5599 < 0.001 16308 0.001
CXCL10/IP-10 39.7 3274 836 0.001 1436 0.001
IL-1α 37.7 42.3 29.9 0.036 32.0 0.065
IL-1β 31.9 135.3 118.1 0.195 100.8 0.012
IL-6 38.4 10846 5969 0.001 8876 0.122
IL-1Ra 31.9 1.1 1.4 0.381 1.7 0.111
IL-1RI 27.1 1.7 0.9 0.001 1.7 0.885
TNF-α 34.5 319.2 705.1 0.001 567.8 0.003
iNOS 35.4 17.6 22.4 0.139 21.2 0.236
COX-2 34.4 23.4 19.0 0.080 35.3 0.005
LIF 30.1 64.8 53.9 0.118 42.1 0.005
  1. IL-1β -stimulated cells were cultured with 31.3 - 1000 mg/L RHP or 1.2 - 38.7 mg/L of GLGPG for 4 h and gene expression was quantified by RT-PCR. Fold changes were calculated as indicated in Materials and Methods. Only the effects of 250 mg/L RHP and 9.7 mg/L GLGPG are shown.
  2. aCT = cycle threshold (CT < 22 = high basal gene expression; 23 < CT < 30 = intermediate basal gene expression; CT > 30 = low basal gene expression).
  3. b p value = significance value between (IL-1β)- and (IL-1β + substance) -treatment.