Skip to main content

Table 4 Effect of RHP and GLGPG on gene expression in human PBL

From: Rose hip and its constituent galactolipids confer cartilage protection by modulating cytokine, and chemokine expression

Gene CT a LPS/IFN-γ LPS/IFN-γ + RHP (250 mg/L) LPS/IFN-γ + GLGPG (9.7 mg/L)
   fold change fold change p valueb fold change p valueb
COX-2 22.8 8.6 8.6 0.880 6.8 0.16
TNF-α 24.9 16.1 8.9 0.004 12.4 0.04
IL-1α 27.2 17.9 11.3 0.002 16.1 0.37
IL-6 29.8 54.8 43.6 0.254 55.6 0.94
CCL5/RANTES 25.8 1.2 1.2 0.908 1.3 0.45
CXCL10/IP-10 35.5 1444 190 < 0.001 2340 0.04
MIP-2 28.6 16.8 18.7 0.577 15.6 0.66
MIP-3α 28.7 37.6 32.3 0.159 23.1 0.01
5-LOX 25.5 0.8 0.6 0.018 0.8 0.96
MMP-9 27.3 0.8 0.9 0.830 0.8 0.81
  1. LPS/IFN-γ -stimulated cells were cultured with 31.3 - 1000 mg/L RHP or 1.2 - 38.7 mg/L of GLGPG for 2 h and gene expression was quantified by RT-PCR. Fold changes were calculated as indicated in Materials and Methods. Only the effects of 250 mg/L RHP and 9.7 mg/L GLGPG are shown.
  2. aCT = cycle threshold (CT < 25 = high basal gene expression; 25 < CT < 30 = intermediate basal gene expression; CT > 30 = low basal gene expression).
  3. b p value = significance value between (LPS/IFN-γ)- and (LPS/IFN-γ + substance) -treatment