Rose hip and its constituent galactolipids confer cartilage protection by modulating cytokine, and chemokine expression

Table 3 Effect of RHP and GLGPG on the production of chemokines and cytokines in human PBL

Protein	Ratio^a	LPS/IFN-γ	LPS/IFN-γ + RHP (250 mg/L)		LPS/IFN-γ + GLGPG (9.7 mg/L)
		pg/mL ± SD	pg/mL ± SD	p value^b	pg/mL ± SD	p value^b
Eotaxin	28	84 ± 2	62 ± 6	0.037	62 ± 3	0.013
MCP-1	497	2940 ± 226	4990 ± 71	0.007	1880 ± 368	0.074
MIP-1β	169	90900 ± 1838	109009 ± 1823	0.005	47550 ± 3465	0.004
MIP-1α	2092	21079 ± 1131	27510 ± 849	0.023	10950 ± 71	0.006
IL-8	794	325103 ± 12738	433040 ± 122728	0.059	267052 ± 36770	0.255
CCL5/RANTES	14	4470 ± 57	1011 ± 55	< 0.001	3210 ± 750	0.067
CXCL10/IP-10	47	12650 ± 16	150 ± 16	0.002	5025 ± 148	0.005
IL-1β	> 2000	21900 ± 2121	14350 ± 1344	0.051	9195 ± 106	0.014
IL-6	> 2000	79650 ± 4031	68450 ± 4031	0.083	38604 ± 2828	0.005
IL-12(p70)	> 200	295 ± 2	19 ± 2	0.002	69 ± 17	0.006
IL-10	67	233 ± 8	382 ± 3	0.002	143 ± 47	0.118
TNF-α	> 2000	21107 ± 990	6595 ± 290	0.003	5105 ± 1775	0.008
IFN-γ	672	2065 ± 106	226 ± 18	0.002	475 ± 2	0.002
GM-CSF	158	148 ± 5	190 ± 47	0.329	54 ± 2	0.002

LPS/IFN-γ -stimulated cells were cultured with 31.3 - 1000 mg/L RHP or 1.2 - 38.7 mg/L of GLGPG for 24 h and proteins were quantified by multiparametric analysis (see Materials and Methods). Only the effects of 250 mg/L RHP and 9.7 mg/L GLGPG are shown.
^a protein secreted by stimulated/protein secreted by unstimulated cells
^b p value = significance value between (LPS/IFN-γ)- and (LPS/IFN-γ + substance) -treatment.

ISSN: 2662-7671