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Table 2 Modulation of gene expression in RAW264.7 cells by RHP and GLGPG

From: Rose hip and its constituent galactolipids confer cartilage protection by modulating cytokine, and chemokine expression

Gene CT a LPS LPS + RHP (250 mg/L) LPS + GLGPG (9.7 mg/L)
   fold change fold change p valueb fold change p valueb
TNF-α 22.9 29.6 21.8 0.0001 20.4 0.002
COX-2 27.5 247 203.3 0.347 217 0.660
PGES 33.0 2.2 1.6 0.0107 2.0 0.214
iNOS 29.9 197 239.4 0.041 224 0.357
IL-1α 33.7 22081 8128 0.0115 18501 0.087
IL-10 38.3 66.8 176 < 0.0001 78.6 0.074
CCL5/RANTES 29.7 515 121 < 0.0001 403 0.003
CXCL10/IP-10 27.2 342 65.4 0.0001 239 0.021
NF-κB1 24.8 6.3 2.1 0.0002 6.0 0.366
NF-κB49 25.3 4.1 1.5 0.0028 3.7 0.028
NF-κBp65 23.6 1.2 0.4 0.0045 1.1 0.126
I-κBα 24.4 5.5 2.0 0.0004 5.7 0.391
CD14 24.9 1.0 0.5 0.198 1.4 0.012
MMP-9 29.0 6.8 3.0 0.0493 7.8 0.145
  1. LPS-stimulated cells were cultured with 31.3 - 1000 mg/L RHP or 1.2 - 38.7 mg/L of GLGPG for 4 h and gene expression was quantified by RT-PCR. Fold changes were calculated as indicated in Materials and Methods. Only the effects of 250 mg/L RHP and 9.7 mg/L GLGPG are shown.
  2. aCT = cycle threshold (CT < 22 = high basal gene expression; 23 < CT < 30 = intermediate basal gene expression; CT > 30 = low basal gene expression).
  3. b p value = significance value between (LPS)- and (LPS + compound)-treatment.