Figure 2

Cytokine production and NF-κB activation assay after M1, M2, and M8 treatment. Fig A - Human monocytes were isolated and differentiated into macrophages. Macrophages ability to produce interferon-γ (IFNγ) and tumor necrosis factor (TNF-α) when challenged with each highly diluted tincture, in the presence or not of 5 μg/mL LPS was determined after 48 hours on supernatant of cultures using CBA. Fluorescence was detected by flow cytometry, and ANOVA and Tukey test were use to determine statistical differences.*LPS-induced macrophages decreased IFN production after M1 and M8 treament and TNF-α release was decreased by all medications. Fig B - Exponentially growing HT29 p NF-κB-hrGFP cells were cultured for 48 hours in absence or presence of 3 ng/mL TNFα with or without M1, M2, or M8 highly diluted tinctures. Percentage of positive GFP cells was determined by flow cytometery. M1 treatment after TNF stimulation has decreased NF-κB activity, and different concentrations of M1 had the same effects. *p < 0.05