Cytotoxic activity of proteins isolated from extracts of Corydalis cava tubers in human cervical carcinoma HeLa cells

Table 2 Defense-related proteins identified in fractions after purification of nucleases from extracts of Corydalis cava tubers using LC-ESI-MS/MS.

No. of protein band^a)	Identified protein^b)	Accesion No.^c)	Matched peptides^d)	Score^e)	Mol. mass (Da)^f)	pI ^g)	Sequence coverage (%)^h)
1, 2, 3, 4,	peroxidase [Nicotiana tabacum] (PR-9)	gi\|14031049	16	560	39495	5.99	13
5, 6	heat shock protein 70 [Chlorella zofingiensis]	gi\|18482472	4	180	70112	8.29	8
1	pectinesterase [Phaseolus vulgaris]	gi\|21060	2	112	23967	9.52	4
2	SODP_PETHY Superoxide dismutase (ISS) [Ostreococcus tauri]	gi\|116056311	2	74	42244	11.68	4
4	GRP-like protein 2 [Gossypium hirsutum]	gi\|110559491	3	69	41333	6.01	8
5	disease resistance protein (TIR-NBS-LRR class), putative [Arabidopsis thaliana]	gi\|15229962	1	64	117364	7.49	0
3	ribosomal protein S12 [Mesostigma viride]	gi\|11466414	1	55	13815	11.32	9
1	DNA-binding protein [Zea mays]	gi\|195658581	1	54	27597	6.49	4
4	chloroplast nucleoid DNA-binding protein-related [Arabidopsis thaliana]	gi\|18391062	1	53	48429	7.48	2
4	RPP13-like protein [Arabidopsis arenosa]	gi\|46410122	1	52	70976	5.51	2

a) Assigned numbers of protein bands as indicated in Fig. 1C.
b) Identified homologous proteins and organism from which it proceeds.
c) Database accession numbers according to: NCBInr (nr); trEMBL (trm).
d) Number of matched peptides with Mascot Search data (http://www.matrixscience.com).
e) Mascot Search Probability Based Mowse Score. Ions score is -10*Log(P), where P is the probability that the observed match is a random event. Individual ions scores > 48 indicate identity or extensive homology (p < 0.05).
f) Theoretical mass (kDa) of identified proteins. The values were retrieved from the protein database.
g) Theoretical pI of identified proteins. The values were retrieved from the protein database.
h) Amino acid sequence coverage for the identified proteins.

ISSN: 2662-7671