Figure 2
Densitometric analysis of open circular and supercoiled DNA damage induced by · OH generated from the Fenton reaction in the presence of plant extracts. Lane 1: untreated control DNA (250 ng); lane 2: FeSO4 (0.5 mM) + H2O2 (25 mM) + DNA (250 ng); lane 3: only H2O2 (25 mM) + DNA (250 ng); lane 4: only FeSO4 (0.5 mM) + DNA (250 ng); lanes 5--15: FeSO4 (0.5 mM) + H2O2 (25 mM) + DNA (250 ng) in the presence of quercetin (1 mM), Gardenia gummifera (0.528 μg/ml), Abies pindrow (0.29 μg/ml), Asphodelus tenuifolius (1.85 μg/ml), Anacyclus pyrethrum (1.52 μg/ml), Orchis mascula (1.60 μg/ml) Holarrhena antidysenterica (0.28 μg/ml), Valeriana wallichii (1 μg/ml), Rosa damascena (0.2 μg/ml), Cleome icosandra (0.16 μg/ml) and Cyperus scariosus (0.128 μg/ml) respectively. Values represent mean ± SD (n = 3). The differences were considered statistically significant if p < 0.05.