Figure 1

Electrophoresis patterns of pBluescript II SK (--) DNA breaks by ^· OH generated from the Fenton reaction and prevented by different plant extracts. (a) Lane 1: untreated control DNA (250 ng); lane 2: FeSO₄ (0.5 mM) + H₂O₂ (25 mM) + DNA (250 ng); lane 3: only H₂O₂ (25 mM) + DNA (250 ng); lane 4: only FeSO₄ (0.5 mM) + DNA (250 ng); lanes 5--10: FeSO₄ (0.5 mM) + H₂O₂ (25 mM) + DNA (250 ng) in the presence of quercetin (1 mM), Gardenia gummifera (0.53 μg/ml), Abies pindrow (0.29 μg/ml), Asphodelus tenuifolius (1.85 μg/ml), Anacyclus pyrethrum (1.52 μg/ml) and Orchis mascula (1.60 μg/ml), respectively (n = 3). (b) Lane 1: untreated control DNA (250 ng); lane 2: FeSO₄ (0.5 mM) + H₂O₂ (25 mM) + DNA (250 ng); lane 3: only H₂O₂ (25 mM) + DNA (250 ng); lane 4: only FeSO₄ (0.5 mM) + DNA (250 ng); lanes 5--10: FeSO₄ (0.5 mM) + H₂O₂ (25 mM) + DNA (250 ng) in the presence of quercetin (1 mM), Holarrhena antidysenterica (0.28 μg/ml), Valeriana wallichii (1 μg/ml), Rosa damascena (0.20 μg/ml), Cleome icosandra (0.16 μg/ml) and Cyperus scariosus (0.13 μg/ml), respectively (n = 3).