Figure 5

Positive staining for IL-1β (a, see densitometry analysis g) was observed in gingivomucosal tissue after ligature mainly localized in the epidermis and in inflammatory cells in derma In gingivomucosal tissue of Hypericum-treated rats no positive staining was observed for IL-1β (b, see densitometry analysis g). Moreover 8 days following ligation, positive staining for ICAM-1 and P-selectin, were found in the gingivomucosal tissues from ligature operated rats (c and e respectively, see densitometry analysis g). In contrast, a positive staining for ICAM-1 and P-selectin was significantly attenuated by the treatment with Hypericum (d and f respectively, see densitometry analysis g). Figure is representative of at least 3 experiments performed on different experimental days. Densitometry data are expressed as % of total tissue area. Data are means of mean ± s.e.m. from N = 10 rats for each group. *P < 0.01 vs. non-ligated. ° < b0.01 vs. ligated.