Fractionation by preparative HPLC of the Ca
-inducing activity in E. purpurea root ethanol extracts. HPLC-separated fractions from 95% ethanol extract of E. purpurea root induce significantly different levels of Ca2+ response. Fraction numbers refer to the time (in minutes) when each fraction eluted from the HPLC column. Bar graphs show the mean values of the transient increase in cytosolic Ca2+ concentration evoked by different fractions (mean ± SEM, n = 3, from three independent experiments). There was no detectable response from fractions #2-67 and fraction #87. Different letters indicate statistically significant differences (p < 0.05) between the cytosolic Ca2+ increase of the six active fractions by one-way analysis of variance followed by the Tukey test.