Thin layer chromatography | ||||||
---|---|---|---|---|---|---|
Extract | Solvent System | No. of spots | Colour in Daylight | Colour in UV | Colour after spraying with Dragendorff | Rfvalue |
Aqueous ethanol | Methanol: chloroform (3 : 7) | 2 | Colourless | Light green fluorescent | Reddish brown | 0.78 |
 |  |  | colourless | Light green fluorescent | Reddish brown | 0.80 |
Paper chromatography | ||||||
Extract | Solvent System | No. of spots | Colour in daylight | Colour in UV | Colour after spraying with ferric chloride | R f value |
Aqueous ethanol | N-butanol: water: acetic acid (4: 1: 5) | 1 | Colourless | green fluorescent | Blue black colour | 0.21 |