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Table 2 Chromatographic results

From: Antidiarrhoea and toxicological evaluation of the leaf extract of Dissotis rotundifolia triana (Melastomataceae)

Thin layer chromatography

Extract

Solvent System

No. of spots

Colour in Daylight

Colour in UV

Colour after spraying with Dragendorff

Rfvalue

Aqueous ethanol

Methanol: chloroform (3 : 7)

2

Colourless

Light green fluorescent

Reddish brown

0.78

   

colourless

Light green fluorescent

Reddish brown

0.80

Paper chromatography

Extract

Solvent System

No. of spots

Colour in daylight

Colour in UV

Colour after spraying with ferric chloride

R f value

Aqueous ethanol

N-butanol: water: acetic acid (4: 1: 5)

1

Colourless

green fluorescent

Blue black colour

0.21