Figure 6

Essential oil treatment does not inhibit PR8 NP mRNA expression in MDCK cells. After infection of MDCK cells with oil-treated (1:4,000 dilution) and untreated PR8 virus at 4°C or 37°C for 18 h, total RNA was extracted and PR8 NP mRNA expression was assessed by relative end-point RT-PCR (A). Transcript levels of NP normalized relative to the constitutively expressed GAPDH gene (B). The data are representative of three separate experiments.