Skip to main content

Table 3 Antioxidant activity of protein fractions from UF and SFD cacao seeds

From: Antitumor activity against murine lymphoma L5178Y model of proteins from cacao (Theobroma cacao L.) seeds in relation with in vitro antioxidant activity

Sample Unfermented seed   Semi-fermented dry seed  
  ABTS+ assay   ORAC assay ABTS+ assay   ORAC assay
  TEAC (mM Trolox/g protein) IC50 (mg protein/mL) TEAC (mM Trolox/g protein) TEAC (mM Trolox/g protein) IC50 (mg protein/mL) TEAC (mM Trolox/g protein)
Albumin 0.14 ± 0.03 (3000) 3. 14 ± 0.08 0.44 ± 0.05 (70) 0.37 ± 0.01 (1500)* 1.51 ± 0.02* 0.65 ± 0.13 (50) *
Globulin 0.18 ± 0.03 (3000) 2.89 ± 0.08 0.17 ± 0.01 (300) 0.22 ± 0.04 (1500) * 2.21 ± 0.06* 0.29 ± 0.04 (150) *
Glutelin 0.88 ± 0.01 (600)** 0.58 ± 0.04** 1.21 ± 0.11 (70)** 1.32 ± 0.01 (300) *,** 0. 22 ± 0.02*,** 1.10 ± 0.73 (50) *,**
GHS 0.44 ± 0.11 (92) 0.081 ± 0.04 0.65 ± 0.44 (21) 0.44 ± 0.11 (92) * 0.081 ± 0.04* 0.65 ± 0.44 (21) *
  1. TEAC (Trolox equivalent antioxidant capacity), GHS (Reduced glutathione).
  2. Data are expressed as the mean of the results of two experiments realized by duplicate ± S.E.M.
  3. Values in parentheses indicate protein concentration assayed expressed as ppm
  4. * p < 0.05 unfermented seed compared with semifermented dry seed.
  5. ** p < 0.05 when is comparing seed protein fraction regardless cacao process.