Abalone visceral extract inhibits tumor metastasis by modulating Cox-2 and Cox-2 related tumor metastasis markers. (A) Metastasized lung mass were homogenized and Cox-2 mRNA level was measured in each group by quantitative real-time PCR. (B) Protein level of Cox-2 was detected using total cell lysates from each group. β-tubulin was used as loading control. (C) Markers of tumor metastasis were measured by quantitative real-time PCR. Values are means with SE. Data are representative of five times independent experiments. One (*), two (**) or three asterisks (***) indicate p < 0.05, p < 0.01 or p < 0.001, respectively.