Figure 3

Effect of administration of abalone visceral extract on the levels of Cox-2 and angiogenic markers in primary tumor. (A) Primary tumor mass were homogenized and Cox-2 mRNA level was measured in each group. (B) Protein level of Cox-2 was detected using total cell lysates from each group. β-tubulin was used as loading control. (C) Angiogenic markers of tumor growth were measured by quantitative real-time PCR. Values are means with SE. Data are representative of five times independent experiments. One (*), two (**) or three asterisks (***) indicate p < 0.05, p < 0.01 or p < 0.001, respectively.