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Table 1 Oligonucleotide primer sequences used in RT-PCR

From: Momordica charantia (bitter melon) inhibits primary human adipocyte differentiation by modulating adipogenic genes

Target Gene Primer sequence Cycling condition Size (bp)
PPAR γ2 Forward 5'-GCATGGTGCCTTCGCTGATGC-3'
Reverse 5'-AGGCCTGTTGTAGAGCTGGGT-3'
95°C/5 min
95°C/1 min,55°C/1 min
72°C/1 min,72°C/7 min
4°C hold, 33 cycles
340
SREBP-1c Forward 5'-TTGCGCAAGGCCATCGACTACATT-3'
Reverse 5'-ACAAGGGGCTGCTCTGGAAAGGTG-3'
94°C/5 min
94°C/1 min,55°C/1 min
72°C/1 min,72°C/7 min
4°C hold, 26 cycles
238
Perilipin Forward 5'-TGGAGACTGAGGAGAAGAAG-3'
Reverse 5'-ATGTGAGAGGGGAGATGG-3'
94°C/1.5 min
94°C/45 sec,52°C/1.5 min
72°C/1 min,72°C/7 min
4°C hold, 23 cycles
120
Resistin Forward 5'-TGGTGGTGGTGGCTGTCCTGG-3'
Reverse 5'-GAGTGAGATGTGGTCTGGGCG-3'
95°C/2 min
95°C/45 sec,55°C/1.5 min
72°C/45 sec,72°C/7 min
4°C hold, 39 cycles
145
GAPDH Forward 5'-AGTCAGCCGCATCTTCTTTTG-3'
Reverse 5'-CTCCTGGAAGGGAGATGG-3'
Condition adjusted as per the target gene 298