Effect of bitter melon juice on PPARγ mRNA expression. Differentiating preadipocytes treated with varying concentrations of BMJ demonstrate a significant reduction in PPARγ mRNA gene expression. Bar graphs depict the densitometry scans of PPARγ amplicons (348-bp) and are expressed as a ratio to GAPDH amplicon (298-bp) intensity. Data are expressed as a percentage of the control (set as 100%) and the values represent the mean ± SE (n = 6) of three independent experiments analyzed in duplicate. a, b, cMean values with common letters do not differ (p < 0.05).