Figure 2

Particle size distribution of bee pollen from Cistus sp. and Brassica sp. determined by Coulter counter. A Coulter counter with 100 μm aperture (particle size; 2-60 μm, particle counting; 50000) was used for particle analysis. Before the analysis, each sample was diluted with electrolyte solution to the appropriate concentration of particles and sonicated for 5 min.